A3597-42
ASAHL (N-Acylsphingosine Amidohydrolase-like)
Size
£863.00
- SKU:
- A3597-42
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- CHO-cell-derived rhASAHL isoform 1. (aa 29 - 359)
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- ASAHL (N-acylsphingosine-amidohydrolase-like; N-acylethanolamine-hydrolyzine acid amidase; also acid ceramidase-like protein) is a 30kD member of the acid ceramidase family. It is a secreted lysosomal glycoprotein that cleaves fatty acid amides such as palmitoylethanolamine. Human ASAHL is synthesized as a 359 amino acid (aa) preproprecursor that contains a 28 aa signal sequence, a 96 aa prosegment, and a 30-31kD, 235 aa mature region that begins at Cys125. Multiple isoforms likely exist. One isoform shows a deletion of aa 324-359, while another has a two aa substitution for aa 198-359. The human proform (aa 29-359) is 78% aa identical to mouse ASAHL proform. Applications: Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhASAHL is ~ 1ng/well. Western Blot: 0.1-0.2ug/ml detection limit for rhASAHL is ~ 10ng/lane under non-reducing and reducing conditions. Immunoprecipitation: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice