A3596-01

AADACL1 (Arylacetamide deacetylase-like 1)

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£642.00

SKU:: A3596-01
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: A synthetic peptide from the C-terminus of aa-(RTRNSYIKWLDQN).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:1,000. Western Blot: 1ug/ml. A 26-28kD band in Human Brain is detected. reliminary experiments gave an approx 26-28kD band in Human Brain (Amygdala, Cerebellum, Hippocampus) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 49.1kDa according to NP_065843.3. The 26-28kD band was successfully blocked by incubation with the immunizing peptide. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See A3596-01-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

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