A3595-95B

Aryl Hydrocarbon Receptor Nuclear Translocator-like 2 (ARNTL2, Transcription Factor BMAL2, CLIF, MOP9, PASD9)

Size

£448.00

SKU:: A3595-95B
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant corresponding to partial sequence of human ARNTL2.
Clone:: 8C17
Format:: Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Extra Details:: BMAL2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription. Also, in umbilical vein endothelial cells, activates SERPINE1 through E-box sites. This transactivation is inhibited by PER2 and CRY1. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.2-2ug/ml Flow Cytometry: 0.5-2ug/sample; HeLa cells fixed in 2% paraformaldehyde/PBS and then permeablized in 90% methanol. Immunocytochemistry: HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Optimal dilutions to be determined by the researcher. Positive Control: HeLa cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.