A3580-05
Artemin (GFRalpha3-RET Ligand)
Size
£576.00
- SKU:
- A3580-05
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human Artemin, expressed in E.coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
- Extra Details:
- Artemin is a novel member of the GDNF family and demonstrated that it is the ligand for the former orphan receptor GFRalpha3-RET. Artemin is a survival factor for sensory and sympathetic neurons in culture, and its expression pattern suggests that it also influences these neurons in vivo. Artemin can also activate the GFRalpha1-RET complex and supports the survival of dopaminergic midbrain neurons in culture Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Sandwich): 0.5-2ug/ml (100ul/well antibody solution). Used in conjunction with A3580-05A, allows the detection of at least 2000-4000pg/well of recombinant human Artemin Western Blot: 0.1-0.2ug/ml. Detection limit for recombinant human Artemin is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice