A3578-BIOTIN

ART2.2 (ADP Ribosyltransferase 2.2) (Biotin)

Size

£960.00

SKU:: A3578-BIOTIN
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: pME.CD8LF-ART2.2.
Clone:: Nika102
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: ART2.2 is expressed exclusively by mature T-cells. Levels of ART2.2 expression vary between inbred strains of mice and is absent in NZW mice, in which the ART2.2 gene is deleted (1). Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized wistar rats were fused with cells of the Sp2/0 mouse myeloma cell line. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.