A3577-01E-PE

Arrestin 1, beta (ARRB1, ARB1, ARR1) (PE)

Size

£914.00

SKU:: A3577-01E-PE
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to the C-terminal region of human beta-arrestin 1, which shares 84% sequence homology with mouse and rat.
Clone:: 7G45
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with R-Phycoerythrin (PE).
Extra Details:: b-arrestin 1 (ARRB1, ARB1, ARR1) is a member of the arrestin protein family, which also includes visual arrestin, cone arrestin and b-arrestin 2. b-arrestins bind phosphorylated heptahelical G protein coupled receptors to desensitize and target them to clathrin-coated pits for endocytosis. b-arrestin 1 acts as a cofactor in the b-adrenergic receptor kinase (BARK)-mediated desensitization of b-adrenergic receptors, resulting in specific dampening of cellular responses to stimuli such as hormones, neurotransmitters or sensory signals (1). b-arrestin 1 exists as two alternately spliced transcripts, encoding two isoforms. Isoform A is a longer variant, while isoform B lacks an alternate in-frame exon compared to isoform A, resulting in a shorter protein (1). The functions of the isoforms have not been fully described. b-arrestin 1 is a cytosolic protein, and is widely expressed in cells, particularly in the central nervous system and in peripheral blood leukocytes (2). Applications: Suitable for use in FLISA and Western Blot. Other applications not tested. Recommended Dilutions: FLISA: 0.1-1ug/ml Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Positive Controls: Mouse RAW264.7 nuclear extracts, rat PC-12 cell lysates. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

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