A3577-01E-BIOTIN

Arrestin 1, beta (ARRB1, ARB1, ARR1) (Biotin)

Size

£914.00

SKU:: A3577-01E-BIOTIN
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to the C-terminal region of human beta-arrestin 1, which shares 84% sequence homology with mouse and rat.
Clone:: 7G45
Format:: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Extra Details:: b-arrestin 1 (ARRB1, ARB1, ARR1) is a member of the arrestin protein family, which also includes visual arrestin, cone arrestin and b-arrestin 2. b-arrestins bind phosphorylated heptahelical G protein coupled receptors to desensitize and target them to clathrin-coated pits for endocytosis. b-arrestin 1 acts as a cofactor in the b-adrenergic receptor kinase (BARK)-mediated desensitization of b-adrenergic receptors, resulting in specific dampening of cellular responses to stimuli such as hormones, neurotransmitters or sensory signals (1). b-arrestin 1 exists as two alternately spliced transcripts, encoding two isoforms. Isoform A is a longer variant, while isoform B lacks an alternate in-frame exon compared to isoform A, resulting in a shorter protein (1). The functions of the isoforms have not been fully described. b-arrestin 1 is a cytosolic protein, and is widely expressed in cells, particularly in the central nervous system and in peripheral blood leukocytes (2). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1ug/ml Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Positive Controls: Mouse RAW264.7 nuclear extracts, rat PC-12 cell lysates. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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