A3576-98
Arrestin 1, beta (ARRB1, ARB1, ARR1)
Size
£837.00
- SKU:
- A3576-98
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- E. coli-derived full-length recombinant human b-Arrestin 1, Met1-Arg418.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- The Arrestin family consists of four members: Arrestin 1 (visual Arrestin), Arrestin 2 (b-Arrestin 1), Arrestin 3 (b-Arrestin 2), and Arrestin 4 (cone Arrestin). While visual and cone Arrestins are found almost exclusively in the retina, b-Arrestins 1 and 2 are ubiquitously expressed, and were initially described as negative regulators of G protein-coupled receptor (GPCR) signaling. More recently, b-Arrestins have been determined to serve as scaffolds for various signaling pathways, including the MAPK cascades activating ERK2, p38a, and JNK3. These b-Arrestin scaffolds tie together the appropriate kinases in series, forming a discreet signaling module that localizes components to specific subcellular environments and facilitates greater kinase activation. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 3-10ug/ml with the appropriate secondary reagents to detect b-Arrestin 1 in paraffin-embedded sections of normal human spleen and human colon cancer. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice