A3550-07C
Arginine, symmetric, dimethyl (SYM10) (Not for Export EU)
Size
£891.00
- SKU:
- A3550-07C
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to four symmetrical dimethyl-arginine-glycine repeats (KR*GR*GR*GR*G in which (R*) denotes symmetric dimethylation of arginine). Conjugated to KLH.
- Format:
- Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
- Extra Details:
- Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: A 1:500-1:1000 detects methylated p60 (unidentified protein) and Sm protein B, B’ and D in RIPA lysates from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transferred to PVDF and probed with A3550-07C (1:1000). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Detects bands at 60, 30, 32 and 16kD, corresponding to p60, Sm B, B’ and D, respectively. Immunoprecipitation: Reported by an independent laboratory to immunoprecipitate proteins containing symmetric dimethyl-arginine. Immunocytochemistry: 1:500. Reported by an independent laboratory to immunostain Cajal bodies in the nuclei of HeLa, WI-38 and NIH/3T3 cells fixed with paraformaldehyde and permeabilized with triton. Optimal dilutions to be determined by the researcher. Included Positive Antigen Control: J9000: Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice