ARC (NT)

Size

£655.00

SKU:: A3325-01
Application:: WB
Purity:: Immunoaffinity purified IgG
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Peptide corresponding to amino acids 2 to 18 of human origin (1).
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK BCL10, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD (1). ARC interacts with caspase-2 and –8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1, and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue (1). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500 Optimal dilutions to be determined by the researcher. Positive Control: Whole cell lysate from HeLa cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.