A2999-50C
Aquaporin 1 (AQP1, CHIP-28, Channel-forming Integral Protein)
Size
£780.00
- SKU:
- A2999-50C
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa249-269 of rat aquaporin 1.
- Clone:
- 3H551 (1/A5F6)
- Format:
- Supplied as a liquid in PBS, 0.1% BSA, 0.09% sodium azide.
- Extra Details:
- Aquaporin 1 is a 28kD intergral membrane protein which was originally identified in red blood cells and the kidney. AQP1 is also expressd by the choroid plexus and various other tissues. The glycosylated forms of AQP1 range between 40-60kD in mass. Applications: Suitable for use in Immunohistochemistry, ELISA, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen and paraffin): 1:500-1:1000, Note: Antigen retrieval using heat treatment prior to staining of paraffin section is required. Sodium citrate buffer pH 6.0 is recommended for this purpose. ELISA: 1:1000-1:20,000 Western Blot: 1:1000-1:5000 Optimal working dilutions to be determined by researcher. Recommended Positive Control: Kidney Hybridoma: Spleen cells from immunized Balb/c mice were fused with the cells of Sp-2/0 Ag14 mouse myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice