A2299-77G
Apolipoprotein H (beta 2 Glycoprotein 1, ApoHb2gp1)
Size
£307.00
- SKU:
- A2299-77G
- Purity:
- Purified by caprylic acid and ammonium sulfate precipitations, and solid -phase adsorption to remove unwanted crossreactivity.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Purified Apolipoprotein-H (beta2-Glycoprotein-I) from human plasma.
- Format:
- Supplied as a liquid in 10mM HEPES, pH 7.4, 150mM sodium chloride and 50% glycerol. No preservatives added.
- Extra Details:
- Apolipoprotein-H, also known as beta2-Glycoprotein I (beta2GPI), is a plasma glycoprotein that circulates at a concentration of 200ug/ml (4uM). Synthesized in the liver, beta2GPI is a single chain molecule of 48kD, consisting of five repeating internally disulphide-bonded structures referred to as sushi domains. Relative to other glycoproteins, beta2GPI has an unusually high content of cysteine (6.2%), proline (8.3%) and carbohydrate (19%). Almost half the circulating b2GPI in plasma is associated with lipoproteins of all major fractions. beta2GPI has been demonstrated to bind negatively charged phospholipids, heparin and platelets. Although the precise function(s) are as yet unknown, beta2GPI has been demonstrated to interfere with blood coagulation by competitively binding to negatively charged phospholipid surfaces exposed during cell activation or damage. Recent evidence also implicates beta2GPI as a cofactor recognized by anti-phospholipid antibodies present in some autoimmune disorders such as systemic lupus erythematosus (SLE). Applications: Suitable for use in ELISA, Immunodiffusion and Immunoelectrophoresis. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice