A2299-31E
Apolipoprotein A-II (Apo A-II)
Size
£812.00
- SKU:
- A2299-31E
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Gln24-Gln100 of human ApoA2 expressed in E. coli. Species Sequence Homology: chimpanzee; 96%, bovine, equine, mouse, and rat; 48%-66%.
- Clone:
- 7H205
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Apolipoprotein A2 (ApoA2) is a major protein component of serum HDL. It is produced by the liver and is involved in cholesteryl ester formation and cholesterol transport from tissues to the liver. Polymorphisms of ApoA2 are associated with disorders of cholesterol and fatty acid metabolism. ApoA2 can form disulfide-linked 17kD homodimers and heterodimers with other apolipoproteins. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml. The detection limit is ~5ng/lane under reducing and non-reducing conditions. Direct ELISA: 0.5-1ug/ml. The detection limit is ~5ng/well. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice