A2298-74M3

APEX2 (AP Endonuclease XTH2, APEX Nuclease-like 2, APEX Nuclease 2, Apurinic-apyrimidinic Endonuclease 2, AP Endonuclease 2, DNA-(apurinic or apyrimidinic site) Lyase 2, APE2, APEXL2, XTH2)

Size

£576.00

SKU:: A2298-74M3
Application:: WB
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptides corresponding to EELRALDSEGRALLTQHKI (aa122-140) and GQAKTSEAKDEKELRTSF (aa438-455) of human APEXL2. Species sequence homology: mouse and rat.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: Apurinic:apyrimidinic (AP) sites occur frequently in DNA by spontaneous hydrolysis, DNADmaging agents or DNA glycosylases that remove specific abnormal bases. ClassII AP endonucleases act to repair this damage by cleaving the phosphodiester backbone. It is primarily located in the nucleus but is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair (BER). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Positive Control: HeLa Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.