A2298-74C1

APEX1, NT (DNA-(apurinic or apyrimidinic site) lyase, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APEX nuclease, APEN, Protein REF-1, APEX1, APE, APEX, APX, HAP1, REF1)

Size

£696.00

SKU:: A2298-74C1
Purity:: Purified by ammonium sulfate precipitation.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide selected from the N-terminal region of human APEX1 (KLH).
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This protein is the major AP endonuclease in human cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.