A2298-70A
APC (Adenomatous Polyposis coli)
Size
£486.00
- SKU:
- A2298-70A
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa1-433 of the N-terminal fragment of human APC (Adenomutons Polyposis Coli gene Chr 5q) (coupled to maltose binding protein). Cellular Localization: Cytoplasmic and nuclear.
- Clone:
- 4i295
- Format:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Extra Details:
- This gene encodes a tumor suppressor protein that includes among its many intracellular functions one of nuclear export. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Western Blot, ELISA and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:10-1:500 Immunohistochemistry: Paraffin sections Immunoprecipitation:1:10-1:500 Immunofluorescence (IC): 20ug/ml Optimal dilutions to be determined by the researcher. Clinical Relevance: Detection of APC mutants from colon cell lines. Positive Control: Colon cell line HCT116, SW480 and SW837 extracts. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice