A2298-22-PE
Antithrombin III (ATIII) (PE)
Size
£792.00
- SKU:
- A2298-22-PE
- Purity:
- Purified by DEAE chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Human Antithrombin III purified from plasma.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- Antithrombin III (ATIII) is a plasma protein synthesized in the liver. Normal plasma levels are 115-160mg/L. ATIII consists of a single polypeptide chain and has a molecular weight of 58kD. ATIII is a serine protease inhibitor and through this action regulates blood coagulation. ATIII also has a heparin binding site and in the presence of heparin the inhibition of thrombin, factors IXa, Xa and XIa is enhanced 1000-fold. Applications: Suitable for use in Radial Immunodiffusion (RID), Double Diffusion and Immunoelectrophoresis. Other applications have not been tested. Recommended Dilutions: RID: 6ul antiserum/cm2 gel vs. 5ul neat - 1:10 dilution human plasma. Double Diffusion: 10ul antiserum vs. 5ul plasma IEP: 100ul antiserum vs. 5ul plasma gives single arc. Note: The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH > 8.2) is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice