A2295-19

Ankyrin (RFXANK, Regulatory Factor X)

Size

£927.00

SKU:: A2295-19
Purity:: Purified from culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human erythroid cells ankyrin
Clone:: Ank016
Format:: Supplied as a liquid in PBS, pH 7.5, 0.01% sodium azide.
Extra Details:: Ankyrin consists of a single peptide of MW 215kD known to be involved in the attachment of spectrin containing membrane skeleton to the plasma membranes. The cytoplasmic domains of many integral membrane proteins including ion channels and cell adhesion molecules are linked to the actin/spectrin cytoskeletal network by a family of proteins known as ankyrins. Ankyrin belongs to a family of closely related polypeptides associated with the plasma membrane of cells in a variety of cell types (e.g., lymphocytes, platelets, fibroblasts and endothelial cells) and various tissues (e.g., brain, kidney, muscle and many epithelial tissues). Ankyrin molecules contain three distinct domains: an NH2-terminal membrane-binding domain (~89-95kD), a spectrin binding domain (~62kD), and a COOH-terminal domain whose length varies as a result of alternative mRNA splicing. Sequences in the COOH-terminal domain are also the most variable between ankryin family members which include ankyrin R and ankyrin G. Ankryin B or brain-type ankyrin is expressed predominantly as 220kD and 440kD isoforms that are the product of a single gene. Ankyrin G is a marker of axon initial segments, nodes of Ranview, sites of cell-cell contact on epithelial cells and intracellular membranes of some cells. Applications: Suitable for use in Western Blot, ELISA, Immunoprecipitation, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:200; low percentage PAGE gels a must; protein is quite large; proteinase inhibitors recommended or lower level fragments likely; membrane preparations and enhance signals. ELISA: 1:1000 Immunoprecipitation: 1:100 Flow Cytometry: 1:500 Immunocytochemistry: 2% paraformaldehyde fixation. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice