Annexin A11 (ANX11, ANXA11, Calcyclin-associated Annexin 50, CAP50)

Size

£825.00

SKU:: A2295-15K
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: E. coli derived recombinant protein corresponding to Met1-Asp505 from human Annexin A11.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Annexin A11 (ANXA11), also known as Annexin XI, 56K Autoantigen and Calcyclin-associated annexin 50 (CAP50), is a 54.4kD (predicted) member of the Annexin protein family. The Annexins are a family of Calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. The Annexin's have a molecular weight of approximately 35-40kD and consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C terminal domain is comprised of four 60-70 amino acid (aa) repeats, known as annexin repeats or an endonexin fold (Annexin A6 contains 8 annexin repeats). The four annexin repeats form a highly α-helical, tightly packed disc known as the annexin domain, which binds to phospholipids in the membrane in a calcium-dependent manner. Members of the annexin family play a role in cytoskeletal interactions, phospholipase inhibition, regulation of cellular growth, and intracellular signal transduction pathways. Annexin A11 was identified as a 56 kD antigen recognized by sera from patients with autoimmune diseases such as rheumatoid arthritis, systemic lupus erythematosus, or Sjogren's syndrome. Human Annexin A11 shares 93% identity with mouse and 49% identity with rat Annexin A11. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml, reducing conditions with lysates of A431 human epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice