A2295-09A-ML650

Angiotensin Converting Enzyme (ACE, CD143) (MaxLight 650)

Size

£1,023.00

SKU:: A2295-09A-ML650
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Angiotensin-converting enzyme from human lung
Clone:: i2H5
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Extra Details:: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Applications: Immunohistochemistry (1,2,3) : 1:1,000 on fresh frozen or 4% paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue. Immunocytochemistry: 1:100. Reacts with endothelial cells, macrophages (activated) and THP1 cells (monocyte line). Immunoprecipitation: 1:1,000 FLISA (4,5): 1:1,000 Structure-function of ACE (6) Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.