A2295-02S2

Angiotensin Converting Enzyme 2, Ectodomain (ACE2, ACE-2, ACE-related Carboxypeptidase, Angiotensin Converting Enzyme Homolog, ACEH, DKFZP434A014, EC 3.4.17, OTTHUMP00000022963)

Size

£895.00

SKU:: A2295-02S2
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to Gln18-Ser740 of human Angiotensin Converting Enzyme 2 ectodomain, expressed in NS0 cells
Format:: Supplied as a lyophilized powder from PBS, 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Flow cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using human, mouse and rat kidney tissue Flow Cytometry: 0.25ug/10e6 cells using HEK293 human cell line transfected with human ACE2 and eGFP Immunohistochemistry (Paraffin): 3-15ug/ml using fixed, frozen sectiond of rat lung, immersion fixed paraffin-embedded sections of human kidney, hamster lung, rat lung and rat kidney Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human Angiotensin Converting Enzyme 2. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.