A2295-02S1
Angiotensin Converting Enzyme 2 (ACE-2, ACEH) Ectodomain
Size
£1,042.00
- SKU:
- A2295-02S1
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gln18-Ser740 of human ACE-2 expressed in NS0 cells.
- Clone:
- 9H63
- Format:
- Supplied as a lyophilized powder from PBS, pH 6.2, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Extra Details:
- Angiogensin I Converting Enzyme-2 (ACE-2) is a type I transmembrane zinc protease that cleaves angiotensins I and II to produce vasodilatory and anti-proliferative peptides. The balance between ACE-1 and ACE-2 activity is critical for maintaining cardiovascular, renal, and pulmonary function. ACE-2 isoforms of 75kD and 120kD are differentially expressed between lung and kidney, respectively, and a shed soluble form is generated by TACE/ADAM17 mediated cleavage. ACE-2 also functions as the cellular uptake receptor for the SARS coronoavirus. Within the extracellular domain, human ACE-2 shares 83% aa sequence identity with mouse and rat ACE-2. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml in NS0 mouse myeloma cell line and human kidney tissue Immunohistochemistry: 8-25ug/ml in immersion fixed paraffin-embedded sections of human kidney and hamster lung. Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma with B cells obtained from a mouse. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice