Angiopoietin-like 3 (ANGPTL3, ANGPT5) (Biotin)

Size

£984.00

SKU:: A2293-48D
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Sf21-derived rhANGPLT3 (Ser17-Glu460). Species sequence homology: 76%, mouse; 83%, porcine.
Format:: Supplied as a lyophilized powder in PBS, BSA. Reconstitute with 1ml sterile TBS, 0.1% BSA. Labeled with Biotin.
Extra Details:: Angiopoietin-like 3 (ANGPTL3), also known as angiopoietin-5, is a secreted, hepatic angiopoietin-like glycoprotein that is structurally related to angiopoietins. Human ANGPTL3 contains an N-terminal heparin-binding motif, a coiled-coil domain (CCD) that mediates the formation of non-covalent higher-order oligomers, and a C-terminal fibrinogen-like domain that binds integrin alphavbeta3. In vivo, ANGPTL3 is proteolytically cleaved between the CCD and the fibrinogen-like domain, generating an N-terminal fragment that retains the inhibitory activity on lipoprotein lipase and a C-terminal fragment that can induce angiogenesis. Full length and cleaved ANGPTL3 circulate in plasma. Mature human ANGPTL3 shares 76% and 83% aa sequence identity with mouse and porcine ANGPTL3, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/ml Detection limit for rhANGPLT3 is ~1ng/lane under non-reducing and reducing conditions. In this format, A2293-48D shows ~20% cross-reactivity with rmANGPTL3. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile TBS, 0.1% BSA. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice