A2281
Androgen Receptor
Size
£837.00
- SKU:
- A2281
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa299-315 (STEDTAEYSPFKGGYTK) of human Androgen Receptor. Cellular Localization: Nuclear. MW of antigen: 110kD
- Clone:
- 0.N.15 (AR 441)
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
- Extra Details:
- The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed as a marker of hormone-responsiveness. Applications: Suitable for use in Gel Supershift, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunoprecipitation (Denatured): 2ug antibody/mg protein lysate (Use Protein G). Western Blot: 1ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:50 for 30 min. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min, followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: LnCap cells or prostate carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice