A2275-87E-HRP
β-Amyloid Precursor Protein (695aa) (HRP)
Size
£971.00
- SKU:
- A2275-87E-HRP
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- rec b-APP695
- Clone:
- 6A6
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- There are 3 known b-APP proteins (b-APP695, b-APP751, b-APP770). The amyloid b-peptide is thought to be derived from all three precursor proteins. Evidence indicates that b-APP is present in senile plaques, extraneuronal tangles, neurous, satellite glial cells of the dorsal root, enteric and trigerminal ganglia, megakaryocytes, cardiac muscle and the pituitary and adrenal glands. Applications: Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Frozen and Paraffin-embedded): 5-10ug/ml. Suitable for immunohistochemical staining of alcohol-fixed paraffin-embedded or frozen tissue sections. Other fixatives may also work following formic acid treatment. Western Blot: 0.5-1.0ug/ml ELISA: 0.1-0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice