A2275-77A

β-Amyloid Peptide

Size

£767.00

SKU:: A2275-77A
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A 30 amino acid synthetic peptide derived from the full length (1-43 amino acid) b-Amyloid peptide.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Extra Details:: The progressive deposition of insoluble amyloid protein plaques in speciﬁc areas of the brain and its blood vessels is associated with a number of neuropathologies and is an early invariant feature of Alzheimer’s disease. The major component of these protein plaques is a peptide of 39-42 amino acids derived from a group of 695-770 amino acid precursor proteins collectively referred to as the b-amyloid precursor protein (Beta-APP). b-APP is highly conserved in vertebrates; however, its normal function has not been well deﬁned. In each of the b-APPs, the b-Amyloid peptide (b-AP) is an internal peptide that begins about 99 residues from the carboxy-terminal end of the b-APP and extends from the extracellular domain into the middle of the hydrophobic, membrane spanning domain of the precursor protein. The precise mechanism whereby b-AP accumulates in brain remains poorly deﬁned. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1ug/ml Western Blot: 1-3ug/ml Immunoprecipitation: 5-10ug; For immunoprecipitation of b-Amyloid peptide from cultured cells, it is necessary to mix the culture medium with an equal volume of 2X RIPA buffer. Immunohistochemistry: 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice