A2275-77A-PE
β-Amyloid Peptide-PE
Size
£1,023.00
- SKU:
- A2275-77A-PE
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- A 30 amino acid synthetic peptide derived from the full length (1-43 amino acid) b-Amyloid peptide.
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- The progressive deposition of insoluble amyloid protein plaques in specific areas of the brain and its blood vessels is associated with a number of neuropathologies and is an early invariant feature of Alzheimer’s disease. The major component of these protein plaques is a peptide of 39-42 amino acids derived from a group of 695-770 amino acid precursor proteins collectively referred to as the b-amyloid precursor protein (Beta-APP). b-APP is highly conserved in vertebrates; however, its normal function has not been well defined. In each of the b-APPs, the b-Amyloid peptide (b-AP) is an internal peptide that begins about 99 residues from the carboxy-terminal end of the b-APP and extends from the extracellular domain into the middle of the hydrophobic, membrane spanning domain of the precursor protein. The precise mechanism whereby b-AP accumulates in brain remains poorly defined. Applications: Suitable for use in FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: FLISA: 0.1-1ug/ml Western Blot: 1-3ug/ml Immunoprecipitation: 5-10ug; For immunoprecipitation of b-Amyloid peptide from cultured cells, it is necessary to mix the culture medium with an equal volume of 2X RIPA buffer. Immunohistochemistry: 5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice