A2275-73C-APC

β-Amyloid, NT (APC)

Size

£953.00

SKU:: A2275-73C-APC
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to the N-terminal region of human amyloid-b
Clone:: NAB228
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: Amyloid-b precursor protein (APP) is the source of the amyloid-beta (Ab) peptide found in neuritic plaques of Alzheimer’s disease (AD) patients. APP is a glycosylated transmembrane protein with a long extracellular N-terminal domain, a short intracellular C-terminal domain, and an Ab-segment of intermediate length shared between the intra- and extracellular regions.1 APP is cleaved by a-secretase immediately before or after reaching the cell surface, releasing the non-amyloidogenic secreted form of the N-terminal soluble APP fragment (sAPPa).2 APP molecules that are not cleaved by a-secretase are internalized, and subjected to b-site cleavage by b-secretase, leaving behind a membrane-bound C-terminal stub.3 This terminal stub is the substrate for g-secretase, which cleaves the molecule at g-site(s) to release the 40 aa, 42 aa, or 43 aa’long Ab peptides.4 Processing of APP by b- and g-secretases occurs under normal physiological conditions, indicating that all fragments of APP, including Ab, play roles in normal physiology.4-5 In neurons, around 95% of APP is cleaved by g-secretases while 5% is cleaved by b-secretase.6 Excessive generation of Ab and its subsequent oligomerization and fibrillation leads to the detrimental effects of AD. Reductions in the level or activity of certain APP fragments, in addition to accumulation of Ab, may play a critical role in the cognitive dysfunction associated with AD, especially in the early stages of the disease. APP fragments including Ab regulate key neural functions including cell excitability, synaptic transmission, long-term potentiation, behavorial learning, and memory.7 Applications: Suitable for use in Immunofluorescence, FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence : 5-10ug/ml FLISA: 0.1-1.0ug/ml Western Blotting: 1-3ug/ml Immunoprecipitation : 10ug/application Immunohistochemistry : 5-10ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice