Amyloid Precursor Protein, CT (APP, Amyloid beta (A4) Precursor Protein) (Not for Export EU)

Size

£825.00

SKU:: A2275-71B
Purity:: Serum
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Nine amino acid peptide from the C-terminus of APP
Format:: Supplied as a liquid, 0.02% sodium azide.
Extra Details:: Amyloid Precursor Protein (APP) is an integral membrane protein with a large ectodomain, a transmembrane domain, and a short cytoplasmic tail. APP is proteolytically cleaved by b-secretase (BACE) to generate the APP N-terminal fragment (sbAPP) with Mr100kD and a C-terminal fragment (C99) with Mr12kD. The smaller 12kD fragment can be further cleaved by g-secretase, an enzyme activity recently attributed to presenilin-2. This second cleavage produces the insoluble b-amyloid (Ab), a peptide of ~4kD. The accumulation of b-amyloid in intracellular neurofibrillary tangles and extracellular plaques is observed in the brains of Alzheimer’s disease patients and Downs’s Syndrome patients. Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: 1:100 Western Blot: 1:500-1:2000. The low abundance of full length APP in untreated cells means that poor response is seen with Western Blot of whole cell lysates. We recommend that membrane fractions be prepared to increase the loading of APP as well as remove potential interferences from soluable proteins. NTera2, Cos7 and Hela cell membrane preparations are positive by Western Blots. Immunoprecipitation: 1:100-1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice