Amyloid beta A3 Precursor Protein (APBA3, X11-γ, Adaptor protein X11 gamma, Mint3, X11L2)

Size

£876.00

SKU:: A2275-61H
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Met1-Leu138 from human APBA3, expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul PBS.
Extra Details:: X11-γ (Adaptor protein X11 gamma; also APBA3, Mint3 and X11L2) is a member of the X11 family of adaptor proteins. Unlike X11-α and -β which are generally neuronal proteins, X11-γ is widely expressed in all tissues. X11-γ has many functions, including MT-MMP and Furin intracellular trafficking and APP binding. Human X11-γ is 575 amino acids (aa) in length. It contains a poly-Ser segment (aa165-171), a PID region (aa217-381) that binds β-amyloid precursor, and two PDZ domains (aa393-478 and 485-560) that bind Arf GTPases. There is one potential splice variant that shows a 34 aa insertion after Ile505. Over aa1-138, human X11-γ shares 62% aa identity with mouse X11-γ. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml detects APBA3 in lysates of HeLa human cervical epithelial carcinoma cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice