A2275-60B5
Amyloid A, Serum (Serum Amyloid A, SAA)
Size
£927.00
- SKU:
- A2275-60B5
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to human SAA protein.
- Clone:
- 11C77
- Format:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
- Extra Details:
- Human Serum Amyloid A protein-1 (SAA1) is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12kD, 104aa, nonglycosylated polypeptide that displaces apoA1 in the HDL3 complex. The SAA1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA1 gene product differs from the SAA2 gene product by only 7aa. Circulating SAA1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three aas being cleaved from either the N- or C-terminus. Mature human SAA1 is 72%, 82% and 72% aa identical to mature mouse, rabbit and hamster SAA1, respectively. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:5,000 ELISA: 1:5,000-1:20,000 Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice