A1475-01P4
AMP Activated Protein Kinase alpha1 (AMPK a1) (Drosophila)
Size
£570.00
- SKU:
- A1475-01P4
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to 29aa within the N-terminal catalytic domain of Drosophila AMPK alpha. Species Sequence Homology: Vertebrates, Human, Mouse AND Rat
- Clone:
- 10B156 (84.1)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- AMPK is a highly conserved heterotrimeric protein kinase found in all eukaryotes. The heterotrimeric complex exists as multiple isoforms and consists of a catalytic alpha subunit, which is stabilised by non-catalytic regulatory beta- and gamma- subunits. AMPK plays a vital role in maintaining cellular energy homeostasis and is activated by rising levels of AMP under conditions of hypoxia, ischemia, exercise, low glucose levels. AMPK may play an important role in the control of type 2 diabetes and metabolic syndromes. Recent studies suggest that AMPK functions as a suppressor of cell proliferation of both malignant and normal cells. Applications: Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:100-1:1,000. Detects a band of ~63kD in rat brain cell lysates. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice