A1379-47
Aminopeptidase O (AP-O, ONPEP)
Size
£819.00
- SKU:
- A1379-47
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corrresponding to Met1-Phe819 from Aminopeptidase O isoform 1, expressed in Sf 21 cells. Species sequence homology: mouse, 76%.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Aminopeptidase O (AP-O; also C9orf3) is a 93kD (predicted), intracellular member of the M1 metalloprotease family of enzymes. It is expressed in heart, testis and pancreas where it apparently cleaves N-terminal Arg and Asn residues. Human AP-O is 819 amino acids in length. It contains one catalytic domain (aa 167-589) with a Zn-binding site (His479-His483) and a C-terminal ARM region (aa 706-819). At least three additional isoforms exist. One shows a deletion of aa 455-553; a second has an 11 aa substitution for the C-terminal 265 amino acids; and a third shows both a deletion of aa 455-553 and an 11 aa substitution for the C-terminal 46 amino acids. Applications: Suitable for use in Direct ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml using recombinant Human Aminopeptidase O/ONPEP Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with Recombinant Human Aminopeptidase O/ONPEP, Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human placenta. Antigen retrieval is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice