A1374-06C
Aminoacylase 1 (ACY1)
Size
£761.00
- SKU:
- A1374-06C
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Purified, insect cell-derived, recombinant human aminoacylase (aa2-408).
- Clone:
- 9L801
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Aminoacylase (ACY1) is an intracellular soluble homodimeric zinc-binding enzyme. It is involved in the breakdown of acetylated amino acids generated during protein degradation by catalyzing the formation of free aliphatic amino acids from N-acetylated precursors. Mutations in the ACY1 gene might cause inborn metabolic disorders. The aa sequence of human ACY1 is 100%, 88% and 86% identical to that of chimpanzee, dog and mouse/rat. Applications: Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml Immunoprecipitation: 25ug/ml Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Hybridoma: Mouse myeloma cells with B cells obtained from a rat immunized with purified, insect cell-derived, recombinant human aminoacylase (aa2-408). Storage and Stability: Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice