A1372-59
AMIGO3 (Amphoterin-Induced Gene and ORF 3, Alivin-3)
Size
£812.00
- SKU:
- A1372-59
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- NS0-derived rmAMIGO3. (aa 20 - 378)
- Clone:
- 8H161
- Format:
- Supplied as a lyophilized powder in 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.
- Extra Details:
- AMIGO3 (Amphoterin-Induced Gene and ORF 3) is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (CRRs) and one Ig domain in their extracellular domains. It is ubiquitously expressed and is detected in all tissues studied. AMIGO3 shows less than 40% amino acid sequence homology with AMIGO1 and 2. The extracellular domain of mouse AMIGO3 shares 90% and 75% amino acid sequence identity with that of rat and human AMIGO3, respectively. Applications: Suitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5- 1.0ug/ml detection limit for rmAMIGO3 is ~ 1ng/well. Western Blot: 1-2ug/ml detection limit for rmAMIGO3 is ~ 25ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 25ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice