A1372-37
AMICA (Adhesion Molecule that Interacts with CXADR Antigen 1, Junction Adhesion Molecule-Like, JAM-L, JAML)
Size
£787.00
- SKU:
- A1372-37
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Leu20-Leu275 of human AMICA, expressed in NS0 cells
- Clone:
- 7H215
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- AMICA (Adhesion Molecule that interacts with CXADR antigen 1), also known as junction adhesion molecule like (JAM-L) is a 60kD type I transmembrane glycoprotein member of the JAM family of adhesion molecules. Human AMICA has an extracellular domain (ECD) of 275 amino acids (aa) and a cytoplasmic tail of 98aa. The ECD contains two Ig-like regions. There is an N-terminal C2-type Ig-like domain and a membrane proximal V-type Ig-like domain. AMICA is expressed by leukocytes and is concentrated at areas of cell-cell contact. It forms noncovalent homodimers on the cell surface and binds CXADR on epithelial cells. Two splice variants apparently exist that only involve the first 40aa of the precursor. Human AMICA ECD is 62% and 76% aa identical to mouse and canine AMICA ECD, respectively. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml. Using a colormetrc detection system the detection limit for recombinant human AMICA is 1ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice