A1355-82
ALKBH2, Recombinant, Human, aa1-261, His-Tag (ABH2, Oxy DC1)
Size
£512.00
- SKU:
- A1355-82
- Molecular Weight:
- 31.4
- Purity:
- ≥90% (SDS-PAGE)
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 1mM DTT, 30% glycerol.
- Extra Details:
- Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, also known ALKBH2, belongs to the ALKB family. Expressed in heart, colon, liver, testis, ovary, prostate and small intestine, ALKBH2 uses iron as a cofactor and functions as a dioxygenase that catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and a-ketoglutarate for enzymatic activity. Source: Recombinant corresponding to aa1-261 from human ALKBH2, fused to His-tag at N-terminus expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K Molecular Weight: ~31.4kD (281aa), confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice