Skip to content
  1. Shop all
  2. Enzymes

A1335-10

Aldolase (Biotin)

Size

£889.00

SKU:
A1335-10
Purity:
Purified by delipidation, fractionation and ion exchange chromatography.
Storage Conditions:
4°C (-20°C Glycerol)
Supplier:
United States Biological
Host:
Goat
Immunogen:
Rabbit muscle aldolase
Format:
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, (IgG and Protease free) 0.01% sodium azide, 40% glycerol. Conjugated with Biotin.
Extra Details:
Applications: Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy. Other applications have not been tested. Recommended Dilution: ELISA: 1:4000 to 1:16,000 using 1.0 ug of Aldolase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. Optimal dilutions to be determined by the researcher. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10–20 BAC molecules per Rabbit Muscle IgG molecule Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice