A1334-70L

Aldo-keto Reductase Family 1 Member C1, Recombinant, Human (AKR1C1, 20-alpha-hydroxysteroid Dehydrogenase, 20-alpha-HSD, 2-alpha-HSD, C9, Chlordecone Reductase Homolog HAKRC, Indanol Dehydrogenase, Dihydrodiol Dehydrogenase 1/2, DD1, DD1/DD2, DDH, DDH1, H-37, HAKRC, High-affinity Hepatic Bile Acid-binding Protein, HBAB, MBAB, MGC8954, Trans-1,2-dihydrobenzene-1,2-diol Dehydrogenase)

Size

£320.00

SKU:: A1334-70L
Application:: WB
Molecular Weight:: 38.9
Purity:: ~90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris HCl pH 8.0, 1mM DTT, 20% glycerol
Extra Details:: AKR1C1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant protein corresponding to aa1-323 of human AKR1C1 6x His tagged expressed in E. coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY Biological Activity: Specific activity is ~0.15-0.2units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH/minute at 25°C. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice