A1125-31A

Akt3 (RAC-PK Gamma)

Size

£825.00

SKU:: A1125-31A
Application:: WB
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: KLH-conjugated, synthetic peptide corresponding to amino acids 119-131 (CSPTSQIDNIGEE) of human Akt3/PKBg. The immunizing sequence is identical in mouse and rat.
Clone:: GMA104
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.
Extra Details:: Applications: Suitable for use in Western Blot and Specificity Assay. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-1ug/ml detects Akt3/PKBg in RIPA lysates from HEK293 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Akt3/PKBg (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Specificity Assay: 1:5,000-1:20,000 dilutions were incubated with various recombinant Akt proteins conjugated to Luminex® microspheres. No crossreactivity with Akt1 or Akt2 was detected. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.