A1125-31A-HRP
Akt3 (RAC-PK Gamma) (HRP)
Size
£1,070.00
- SKU:
- A1125-31A-HRP
- Application:
- WB
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- KLH-conjugated, synthetic peptide corresponding to amino acids 119-131 (CSPTSQIDNIGEE) of human Akt3/PKBg. The immunizing sequence is identical in mouse and rat.
- Clone:
- GMA104
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Applications: Suitable for use in Immunoblot and Specificity Assay. Other applications not tested. Recommended Dilution: Western Blot: 0.1-1ug/ml detects Akt3/PKBg in RIPA lysates from HEK293 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Akt3/PKBg (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Specificity Assay: 1:5,000-1:20,000 dilutions were incubated with various recombinant Akt proteins conjugated to Luminex® microspheres. No crossreactivity with Akt1 or Akt2 was detected. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice