A1125-30L
AKT3 (Protein Kinase B gamma, PKB gamma, PKBG, RAC gamma Serine/Threonine Protein Kinase, RACPK gamma, Serine Threonine Protein Kinase AKT3, STK-2, v-AKT Murine Thymoma Viral Oncogene Homolog 3)
Size
£581.00
- SKU:
- A1125-30L
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to amino acid residues 119-136 (CSPTSQIDNIGEEEMDAS) of human Akt3.
- Clone:
- 66C1247
- Format:
- Supplied as a llquid in PBS, 0.05% BSA, 0.05% sodium azide.
- Extra Details:
- Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha, 2/beta and 3/gamma). Akt1, 2 and 3 contain a key regulatory serine phosphorylation site in the carboxy-terminal region of the protein. Akt/PKB phosphorylation is critical for it activity. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunofluorescence. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:2000 Optimal dilutions to be determined by the researcher. Cellular Localization: Cytoplasmic and membrane-associated after cell stimulation leading to its translocation. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice