A1125-26J

Akt2 (AKT-2, Protein Kinase Akt2, Protein Kinase B beta, PKB beta, PKBBeta, PRKBB, Rac Protein Kinase beta, RAC-BETA, RAC-beta Serine/threonine Protein Kinase, RAC-PK-beta, v-AKT Murine Thymoma Viral Oncogene Homolog 2) (Azide Free)

Size

£483.00

SKU:: A1125-26J
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: A five residue synthetic peptide based on the human Akt2, coupled to KLH.
Format:: Supplied as a liquid in rabbit immunoglobulin in 0.1M Tris-acetate buffer, 0.09% sodium azide.
Extra Details:: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a Pleckstrin Homology (PH) domain which might be important for dimerization of the kinase. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1) and PDK2 (1- 3). Myelin basic protein and histone H2B are in vitro substrates for PKB, while glycogen synthase kinase-3 has been implicated as a physiological target (4). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration (1-3). Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: 13000 dilution of SPC-148 was sufficient for detection of PKBB in 20ug of HeLa cell lysate by ECL immunoblot analysis. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice