A1125-18
Akt1 (Rac PKa, PKBa)
Size
£843.00
- SKU:
- A1125-18
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide, aa466-480 (CRPHFPQFSYSASGTA) corresponding to the C-terminal of rat Akt1/PKBa, with an N-terminal cysteine added for conjugation. Species sequence homology: mouse Akt1-15/15 bovine Akt1-14/15 and human Akt1-13/15
- Clone:
- 4H304 (AW24)
- Format:
- Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
- Extra Details:
- The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:5000-1:40,000 detects Akt1/PKBa in RIPA lysates from human HEK293 cells. HEK293 cell lysate or 40ng of purified Akt1 protein, Akt2 protein and Akt3 protein were resolved by electrophoresis, transferred to nitrocellulose and probed with A1125-18 (1:10,000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5ul immunoprecipitated Akt1/PKBa from 500ug of HEK293 RIPA lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice