A1125-04C
Akt1, phosphorylated (Ser473) (Rac PKa, PKBa)
Size
£614.00
- SKU:
- A1125-04C
- Purity:
- Purified by Protein G affinity chromatography
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic phosphopeptide corresponding to phosphorylated serines at amino acid residue 473 of human Akt1. The sequence in this region is identical in mouse, chicken and frog
- Clone:
- 104A282
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide
- Extra Details:
- Akt, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and Akt/PKB kinase activity. Akt/PKB phosphorylation is critical for it activity. The major phosphorylation sites required for Akt activation has been identified as threoinine 308 and serine 473. Serine 473 is phosphorylated by MAPKAP kinase 2. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:200-1:250 Immunohistochemistry (FFPE): 1:200-1:250 Requires HIER using sodium citrate buffer pH 6.0 prior to staining. Optimal dilutions to be determined by the researcher. Positive Control: PDGF treated NIH 3T3 Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice