A1125-04-ML550
Akt1, PH Domain, Serine-Threonine Phosphorylation (Rac PKa, PKBa) (MaxLight 550)
Size
£1,081.00
- SKU:
- A1125-04-ML550
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GST-fusion protein corresponding to residues 1-149 of human Akt1
- Clone:
- 1.T.1 (SKB1)
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. The serine/threonine kinase Akt1 has also been described as Rac-PKa or PKBa. Rac protein kinase family members feature a pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the carboxy terminus. The PH domain (amino acids 1–106) has been implicated in interactions with other proteins such as G-protein beta gamma subunits, as well as phosphoinositides. The kinase domain is located between residues 148 to 411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells. Akt1 is a protein of 480 amino acids and has been observed to show different migratory patterns on a western blot according to the state of phosphorylation of the protein. Phosphatase treatment has been shown to result in inactivation of the protein. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunocytochemistry, Immunoprecipitation Kinase and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot Analysis: 0.1-1ug/ml detects Akt (60kD) in RIPA lysates from A431 cells. A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A1125-04 (0.5 and 1.0ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4ug immunoprecipitates Akt from 500ug of L6 RIPA lysate. Immunoprecipitation Kinase: 4ug immunoprecipitates active Akt from 700ug of L6 cells stimulated with 15nM IGF-1 for 2-5 min. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice