A1125-03A

Akt1, phosphorylated (Ser473) (Rac PKa, PKBa)

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£832.00

SKU:: A1125-03A
Purity:: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: KLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues (PQFpSYSA) around phosphoserine 473.
Clone:: 11E6
Format:: Supplied as a lyophilized powder in PBS, PEG, sucrose, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Extra Details:: The serine/threonine kinase Akt1 has also been described as Rac-PKa or PKBa. Rac protein kinase family members feature a pleckstrin homology (PH) domain at the amino terminus and a protein-serine/threonine kinase catalytic domain at the carboxy terminus. The PH domain (amino acids 1–106) has been implicated in interactions with other proteins such as G-protein beta gamma subunits, as well as phosphoinositides. The kinase domain is located between residues 148 to 411. These enzymes are activated by diverse ligands such as PGDF, EGF and basic FG in NIH 3T3, Rat-1 or Swiss-3T3 cells. Akt1 is a protein of 480 amino acids and has been observed to show different migratory patterns on a western blot according to the state of phosphorylation of the protein. Phosphatase treatment has been shown to result in inactivation of the protein. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml of this lot detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 100ng/ml PDGF for 20 minutes. Non-treated (lane 1) or PDGF-treated (lane 2) mouse NIH-3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with antiphospho-Akt1/PKBa (Ser473) (0.5ug/ml). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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