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A1125-03

Akt1, phosphorylated (Ser473) (Rac PKa, PKBa)

Size

£754.00

SKU:
A1125-03
Application:
WB
Purity:
Purified by Protein A affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
KLH-conjugated, synthetic peptide containing a pSer that corresponds to amino acid position 473 of human Akt1/PKBa. The immunizing sequence is identical in mouse and bovine Akt1. Akt2 and Akt3 share significant homology with the peptide immunogen sequence.
Clone:
SK703
Format:
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Extra Details:
Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.07-0.4ug/ml of this lot detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 50ng/ml PDGF for 20 minutes. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice