A1125-03-BIOTIN

Akt1, phosphorylated (Ser473) (Rac PKa, PKBa) (Biotin)

Size

£1,023.00

SKU:: A1125-03-BIOTIN
Application:: WB
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: KLH-conjugated, synthetic peptide containing a pSer that corresponds to amino acid position 473 of human Akt1/PKBa. The immunizing sequence is identical in mouse and bovine Akt1. Akt2 and Akt3 share significant homology with the peptide immunogen sequence.
Clone:: SK703
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Extra Details:: Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot Analysis: 0.07-0.4ug/ml of this lot detected phosphorylated Akt1/PKBa in lysates from mouse NIH-3T3 fibroblasts treated with 50ng/ml PDGF for 20 minutes. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.