A1125-01L

Akt1, active, Recombinant, Human, GST-Tag (Rac PKa, PKBa)

Size

£907.00

SKU:: A1125-01L
Molecular Weight:: 60.2
Purity:: ≥70% (SDS-PAGE)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween 20, 3mM DTT, 50% glycerol.
Extra Details:: Human Akt1 (GenBank Accession No. NM_001014432), full length (a.a. 1-480) with N-terminal GST tag, co-expressed with PDK1 in an Baculovirus infected Sf9 cell expression system. Specific Activity: ~12pmol/min/ug Assay Conditons: Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, and 2uM substrate (SER/THR 6) at RT for 1 hour. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.